Article
Isolation and characterization of an oligomerization-negative mutant of HIV-1 integrase.
Virology - 5 Jul 1999
Kalpana G V, Reicin A, Cheng G S, Sorin M, Paik S, Goff S P
Abstract excerpt
The yeast two-hybrid method was used to screen mutagenized DNAs to isolate a variant of the human immunodeficiency virus type 1 integrase (IN) that does not interact with the wild-type IN. The responsible mutation, leading to a single amino acid change (V260E) in the C-terminal domain of IN, blocks IN-IN multimerization but has only small effect on binding to a host interacting protein, INI1 (hSNF5). Binding...
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