Article
Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on integrase functions in vitro.
The Journal of biological chemistry - 25 Jan 1993
Leavitt A D, Shiue L, Varmus H E
Abstract excerpt
The retroviral integrase (IN) protein is essential for integration of retroviral DNA into the host cell genome. To identify functional domains within the protein and to assess the importance of conserved residues, we performed site-directed mutagenesis of HIV-1 IN and analyzed the mutants in vitr...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Codon
- Conserved Sequence
- DNA Nucleotidyltransferases
- DNA, Bacterial
- DNA, Viral
- Gene Expression
- HIV-1
- Integrases
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Structure-Activity Relationship
- Zinc Fingers