Article
Heterologous overexpression of human NEFA and studies on the two EF-hand calcium-binding sites.
Biochemical and biophysical research communications - 24 Jun 1999
Kroll K A, Otte S, Hirschfeld G, Barnikol-Watanabe S, Götz H, Sternbach H, Kratzin H D, Barnikol H U, Hilschmann N
Abstract excerpt
Human NEFA is an EF-hand, leucine zipper protein containing a signal sequence. To confirm the calcium binding capacity of NEFA, recombinant NEFA analogous to the mature protein and mutants with deletions in the EF-hand domain were expressed in Pichia pastoris and secreted into the culture medium at high yield. The calcium binding activity of each purified protein was measured by a modified equilibrium dialysis...
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