Changes in calcium and collagen IV binding caused by mutations in the EF hand and other domains of extracellular matrix protein BM-40 (SPARC, osteonectin).

Abstract excerpt

Recombinant expression in a human kidney cell-line was used to prepare mutant human BM-40 with deletions including the N-terminal acidic domain, a central alpha-helical domain and the C-terminal EF hand domain. Two putative EF hand motifs were altered by point mutations. Only elimination of the whole EF hand domain or its single disulfide bond decreased production and secretion indicating that the C-terminal...

Topics

• Amino Acid Sequence
• Base Sequence
• Calcium
• Cell Line
• Collagen
• Consensus Sequence
• DNA Mutational Analysis
• Glycosylation
• Humans
• Kinetics
• Magnesium