Article
Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies.
The EMBO journal - 1 Jun 1999
Sun Z Y, Dötsch V, Kim M, Li J, Reinherz E L, Wagner G
Abstract excerpt
A general strategy is presented here for producing glycan-free forms of glycoproteins without loss of function by employing apolar-to-polar mutations of surface residues in functionally irrelevant epitopes. The success of this structure-based approach was demonstrated through the expression in Escherichia coli of a soluble 11 kDa adhesion domain extracted from the heavily glycosylated 55 kDa human CD58...
Topics
- Amino Acid Sequence
- Binding Sites
- CD2 Antigens
- CD58 Antigens
- Escherichia coli
- Glycosylation
- Humans
- Models, Molecular
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Peptide Fragments
- Polysaccharides