Article
An insertional mutagenesis system for analyzing the Chinese cabbage genome using Agrobacterium T-DNA.
Molecules and cells - 1 Mar 2010
Yu Jae-Gyeong, Lee Gi-Ho, Kim Jung-Sun, Shim Eun-Jo, Park Young-Doo
Abstract excerpt
In this study, we applied insertional mutagenesis using Agrobacterium transfer DNA to functionally characterize the gene of Brassica rapa L. ssp. pekinensis. The specific objectives were to: (i) develop and apply a gene tagging system using plasmid rescue and inverse PCR, (ii) select and analyze...
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