Article
GLABRA2-based selection efficiently enriches Cas9-generated nonchimeric mutants in the T1 generation.
Plant physiology - 5 Oct 2021
Kong Xiangjiu, Pan Wenbo, Sun Nengxu, Zhang Tingyu, Liu Lijing, Zhang Huawei
Abstract excerpt
The CRISPR/Cas9 system is a widely used tool for genome editing in plants. In Arabidopsis (Arabidopsis thaliana), egg cell-specific promoters driving Cas9 expression have been applied to reduce the proportion of T1 transformants that are chimeras; however, this approach generally leads to relatively low mutagenesis rates. In this study, a GLABRA2 mutation-based visible selection (GBVS) system was established to...
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