Article
A high-throughput system for genome-wide measurement of genetic recombination in Arabidopsis thaliana based on transgenic markers.
Functional & integrative genomics - 1 Nov 2000
Barth S, Melchinger A E, Devezi-Savula B, Lübberstedt T
Abstract excerpt
A high-throughput system for the measurement of recombination frequencies in the genetic model plant, Arabidopsis thaliana, is described. It is based on 21 mono-transgenic isogenic lines harboring antibiotic resistance genes on all five chromosomes. Recombination between pairs of gene insertions in repulsion phase that confer resistance against kanamycin (kan) and hygromycin (hyg) is determined by a phenotypic...
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