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Article

Biolistic transformation of Arabidopsis root hairs: a novel technique to facilitate map-based cloning.

The Plant journal : for cell and molecular biology - 1 Aug 2001

Kemp A, Parker J, Grierson C

Abstract excerpt

The final stage of map-based gene isolation is complementation of the mutant phenotype with wild-type DNA to determine the exact location of the gene of interest. This usually involves Agrobacterium tumefaciens-mediated transformation, which is reliable and produces stable transformants. However, the process of Agrobacterium transformation may take up to three months to complete. If the mutant phenotype can be...

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PMID: 11532182
DOI: 10.1046/j.1365-313x.2001.01090.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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