Article
Biolistic transformation of Arabidopsis root hairs: a novel technique to facilitate map-based cloning.
The Plant journal : for cell and molecular biology - 1 Aug 2001
Kemp A, Parker J, Grierson C
Abstract excerpt
The final stage of map-based gene isolation is complementation of the mutant phenotype with wild-type DNA to determine the exact location of the gene of interest. This usually involves Agrobacterium tumefaciens-mediated transformation, which is reliable and produces stable transformants. However,...
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