Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Nonradioactive labeling of large DNA fragments for genome walking, RFLP and northern blot analysis.

BioTechniques - 1 Aug 1999

Adhami F, Müller S, Hauser M T

Abstract excerpt

In this report, we present a simple nonradioactive labeling procedure for DNA fragments of high specific labeling density that can be used for a variety of applications. The protocol is based on the universal mono-functional platinum reagent for chemical digoxigenin (DIG) labeling of nucleic acids. The labeling protocol was optimized for large DNA templates as complete bacterial artificial chromosomes (BAC)....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arabidopsis
Share to Blotting, Northern
Share to Chromosome Walking

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Contig Mapping

Share to Nucleic Acid Hybridization

Share to Polymorphism, Restriction Fragment Length

Share to RNA, Plant

Source

Identifiers

PMID: 10457838
DOI: 10.2144/99272st02

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Nonradioactive labeling of large DNA fragments for genome walking, RFLP and northern blot analysis.PMID 10457838Read ↗

Purification of Arabidopsis DNA for genotyping in 96-well plates using the puregene DNA purification system.Semantic proximity Read ↗

Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes.Semantic proximity Read ↗

A plant cocktail amenable for PCR-based genetic analysis in Arabidopsis thaliana.Semantic proximity Read ↗

Methods for Target Enrichment Sequencing via Probe Capture in Legumes.Semantic proximity Read ↗

Physical mapping of repetitive oligonucleotides facilitates the establishment of a genome map-based karyotype to identify chromosomal variations in peanut.Semantic proximity Read ↗

Target enrichment sequencing in cultivated peanut (Arachis hypogaea L.) using probes designed from transcript sequences.Semantic proximity Read ↗

Target preparation for genotyping specific genes or gene segments.Semantic proximity Read ↗

A method for detecting DNA polymorphism in large populations.Semantic proximity Read ↗

Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning.Semantic proximity Read ↗

Crop plant genotyping by real-time PCR analysis of crude extracts of seeds.Semantic proximity Read ↗

Targeted enrichment strategies for next-generation plant biology.Semantic proximity Read ↗

Real-time polymerase chain reaction (PCR) quantitative detection of Brassica napus using a locked nucleic acid TaqMan probe.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.