Article
Overexpression, purification and characterization of Mycobacterium bovis BCG alcohol dehydrogenase.
European journal of biochemistry - 1 Jun 1999
Wilkin J M, Soetaert K, Stélandre M, Buyssens P, Castillo G, Demoulin V, Bottu G, Laneelle M A, Daffe M, De Bruyn J
Abstract excerpt
A previous study of the effect of zinc deprivation on Mycobacterium bovis BCG pointed out the potential importance of an alcohol dehydrogenase for maintaining the hydrophobic character of the cell envelope. In this report, the effect of the overexpression of the M. bovis BCG alcohol dehydrogenase (ADH) in Mycobacterium smegmatis and M. bovis BCG is described. The purification of the enzyme was performed to...
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