Article
Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus.
Biochimica et biophysica acta - 1 Jan 2014
Meng Demei, Shen Lin, Yang Rui, Zhang Xinhua, Sheng Jiping
Abstract excerpt
BACKGROUND: 1-Aminocyclopropane-1-carboxylate oxidase (ACO) is a key enzyme that catalyses the final step in the biosynthesis of the plant hormone ethylene. Recently, the first ACO homologue gene was isolated in Agaricus bisporus, whereas information concerning the nature of the ethylene-forming activity of this mushroom ACO is currently lacking. METHODS: Recombinant ACO from A. bisporus (Ab-ACO) was purified and...
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