Article
Functional characterization of flavin-containing monooxygenase 1B1 expressed in Saccharomyces cerevisiae and Escherichia coli and analysis of proposed FAD- and membrane-binding domains.
The Journal of biological chemistry - 15 Mar 1993
Lawton M P, Philpot R M
Abstract excerpt
A cDNA encoding the flavin-containing monooxygenase of rabbit lung (FMO 1B1) was expressed in yeast and Escherichia coli and the recombinant enzymes characterized. A high copy, isopropyl-1-thio-beta-D-galactopyranoside (IPTG)-inducible E. coli expression vector, pKKHC, was used for expression in...
Topics
- Animals
- Base Sequence
- Binding Sites
- Catalysis
- Cell Membrane
- Cloning, Molecular
- DNA
- Escherichia coli
- Flavin-Adenine Dinucleotide
- Molecular Sequence Data
- Mutation
- Oxygenases
- Rabbits
- Recombinant Proteins
- Saccharomyces cerevisiae
- Sequence Deletion