Article
Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition.
Journal of bacteriology - 1 May 1999
Spellerberg B, Pohl B, Haase G, Martin S, Weber-Heynemann J, Lütticken R
Abstract excerpt
Streptococcus agalactiae is a poorly transformable bacterium and studies of molecular mechanisms are difficult due to the limitations of genetic tools. Employing the novel pGh9:ISS1 transposition vector we generated plasmid-based mutant libraries of S. agalactiae strains O90R and AC475 by random chromosomal integration. A screen for mutants with a nonhemolytic phenotype on sheep blood agar led to the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
