Protein footprinting at cysteines: probing ATP-modulated contacts in cysteine-substitution mutants of yeast DNA topoisomerase II.

doi:10.1073/pnas.96.9.4862

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Article

Protein footprinting at cysteines: probing ATP-modulated contacts in cysteine-substitution mutants of yeast DNA topoisomerase II.

Proceedings of the National Academy of Sciences of the United States of America - 27 Apr 1999

Tu B P, Wang J C

Abstract excerpt

Cysteine-substitution mutants of yeast DNA topoisomerase II were used to test footprinting of the enzyme by 2-nitro-5-thiocyanobenzoate, which cyanylates exposed cysteines in a native protein for peptide cleavage at the cyanylated sites upon unfolding and incubating the protein at pH 9. For a mutant enzyme containing a single cysteine, the extent of peptide cleavage was found to reflect the accessibility of the...

Topics

Amino Acid Substitution
Cysteine
DNA Footprinting
DNA Topoisomerases, Type II
Fungal Proteins
Mutation
Saccharomyces cerevisiae

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Identifiers

PMID: 10220384
DOI: 10.1073/pnas.96.9.4862
PMCID: PMC21782

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