Article
Introduction of internal cysteines as conformational probes in yeast phosphoglycerate kinase.
Protein engineering - 1 Jan 1990
Ballery N, Minard P, Desmadril M, Betton J M, Perahia D, Mouawad L, Hall L, Yon J M
Abstract excerpt
Several mutants of yeast phosphoglycerate kinase, each containing only one internal cysteine residue, were constructed from a single mutant devoid of cysteine. These cysteines were introduced as local conformational probes in selected buried positions. The enzyme activity, conformational characteristics and stability indicated that the mutations introduced only small perturbations in the molecule. The...
Topics
- Catalysis
- Circular Dichroism
- Cysteine
- Drug Stability
- Guanidine
- Guanidines
- Kinetics
- Molecular Structure
- Mutation
- Phosphoglycerate Kinase
- Protein Conformation