Article
Rapid kinetic studies and structural determination of a cysteine proteinase mutant imply that residue 158 in caricain has a major effect upon the ability of the active site histidine to protonate a dipyridyl probe.
Biochemistry - 26 Nov 1996
Katerelos N A, Goodenough P W
Abstract excerpt
Cysteine proteinases are endopeptidases whose catalytic activity depends upon the nucleophilicity of the active site cysteine thiol group. An ion pair forms with an active site histidine. The presence in some cysteine proteinases of an aspartic acid close to the ion pair has been used as evidence...
Topics
- Binding Sites
- Cysteine Endopeptidases
- Enzyme Activation
- Histidine
- Kinetics
- Models, Molecular
- Mutation
- Plant Proteins
- Protein Conformation
- Recombinant Proteins
- Structure-Activity Relationship