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Article

Thiol-sensitive mutant forms of human SOD2, L60F, and I58T: the role of Cys140.

Free radical biology & medicine - 1 May 2010

Hernandez-Saavedra Daniel, Quijano Celia, Demicheli Verónica, Souza José M, Radi Rafael, McCord Joe M

Abstract excerpt

We previously described a coding mutation (L60F) in the mitochondrial superoxide dismutase (SOD2) gene of the human T cell leukemia-derived cell line Jurkat. In cell extracts the L60F mutant enzyme showed unusual inhibition by thiol reagents not seen in wild-type enzyme. Here we compare the properties of purified recombinant L60F SOD2 with a previously described SOD2 mutant, I58T. Both mutant proteins display a...

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Identifiers

PMID: 20144705
DOI: 10.1016/j.freeradbiomed.2010.02.001

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SOD2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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