Article
Chemical-modification rescue assessed by mass spectrometry demonstrates that gamma-thia-lysine yields the same activity as lysine in aldolase.
Protein science : a publication of the Protein Society - 1 Jul 2002
Hopkins Christopher E, O'Connor Peter B, Allen Karen N, Costello Catherine E, Tolan Dean R
Abstract excerpt
The role of active site residues in fructose 1,6-bisphosphate aldolase is investigated by chemical-modification rescue. An active-site mutation, K107C, is constructed in a background where the four solvent-accessible cysteine residues are converted to alanine. The resulting mutant, tetK107C, when reacted with bromoethylamine (BrEA), shows a 40-fold increase in activity (to 80% that of wild type). Determination of...
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