Article
Basic phenotypic analysis of six novel yeast genes reveals two essential genes and one which affects the growth rate.
Yeast (Chichester, England) - 15 Mar 1999
Sanjuan R, León M, Zueco J, Sentandreu R
Abstract excerpt
Phenotypic analysis was performed on six mutants of Saccharomyces cerevisiae deleted in one of the following open reading frames (ORFs), located on chromosome II: YBR254c, YBR255w, YBR257w, YBR258c, YBR259w and YBR266c. Disruption of the ORFs was carried out in the diploid strain FY1679 using the kanMX4 marker flanked by short sequences homologous to the target locus. Tetrad analysis following sporulation of the...
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