Article
Disruption of six novel yeast genes located on chromosome II reveals one gene essential for vegetative growth and two required for sporulation and conferring hypersensitivity to various chemicals.
Yeast (Chichester, England) - 1 Jul 1999
Kucharczyk R, Gromadka R, Migdalski A, Slonimski P P, Rytka J
Abstract excerpt
A PCR-based method for targeted gene deletion by kanMX4 module was used to construct complete deletion mutants of six individual open reading frames from chromosome II: YBR128c, YBR131w, YBR133c, YBR137w, YBR138c and YBR142w. The ORFs were deleted in two diploid strains, FY1679 and W303. Sporulation and tetrad analysis revealed that only one ORF, YBR142w, encoding a putative DEAD-box RNA helicase, is an essential...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.