Article
Disruption of six Saccharomyces cerevisiae genes from chromosome IV and basic phenotypic analysis of deletion mutants.
Yeast (Chichester, England) - 30 Sept 1998
López M C, Sánchez M, Fermiñán E, Domínguez A
Abstract excerpt
We report here the construction of six deletion mutants and the analysis of their basic phenotype. Deletion cassettes containing the KanMX4 marker module and long flanking regions homologous to the target locus were constructed for each of the six open reading-frames (ORFs YDL088c, YDL087c, YDL08...
Topics
- Cloning, Molecular
- Escherichia coli
- Gene Deletion
- Genes, Fungal
- Genetic Complementation Test
- Kanamycin
- Mutagenesis, Insertional
- Open Reading Frames
- Phenotype
- Polymerase Chain Reaction
- Saccharomyces cerevisiae
- Spores, Fungal