Article
Genomic disruption of six budding yeast genes gives one drastic example of phenotype strain-dependence.
Yeast (Chichester, England) - 1 May 1998
Bilsland E, Dahlén M, Sunnerhagen P
Abstract excerpt
Using PCR to construct disruption cassettes, null alleles of six genes have been created in Saccharomyces cerevisiae. In a FY1679 background, no defects were detected in any of the haploid deletion mutants with respect to growth, gross morphology, or mating. A diploid FY1679-derived delta ygl194c...
Topics
- Chromosome Mapping
- Genes, Fungal
- Microscopy, Fluorescence
- Phenotype
- Plasmids
- Polymerase Chain Reaction
- Saccharomyces cerevisiae
- Sequence Analysis, DNA
- Sequence Deletion
- Structure-Activity Relationship
