Article
A method for selection of forward mutations in supF gene carried by shuttle-vector plasmids.
Carcinogenesis - 1 Feb 1993
Ariza R R, Roldán-Arjona T, Hera C, Pueyo C
Abstract excerpt
The supF gene of Escherichia coli has been widely used as a mutagenic target in several shuttle-vector plasmids. Mutations in this gene are usually screened by a colony colour assay based on the suppression of a lacZ amber mutation in an appropriate bacterial indicator strain. This screening meth...
Topics
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Genes, Suppressor
- Genetic Vectors
- Methylnitrosourea
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Phenotype
- Plasmids
- RNA, Transfer