Article
A method for selection of forward mutations in supF gene carried by shuttle-vector plasmids.
Carcinogenesis - 1 Feb 1993
Ariza R R, Roldán-Arjona T, Hera C, Pueyo C
Abstract excerpt
The supF gene of Escherichia coli has been widely used as a mutagenic target in several shuttle-vector plasmids. Mutations in this gene are usually screened by a colony colour assay based on the suppression of a lacZ amber mutation in an appropriate bacterial indicator strain. This screening method cannot measure the low mutation frequencies usually detected in prokaryotes, and therefore precludes the use of supF...
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