Use of high and low level overexpression plasmids to test mutant alleles of the recF gene of Escherichia coli K-12 for partial activity.

doi:10.1093/genetics/135.3.643

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Article

Use of high and low level overexpression plasmids to test mutant alleles of the recF gene of Escherichia coli K-12 for partial activity.

Genetics - 1 Nov 1993

Sandler S J, Clark A J

Abstract excerpt

We showed that sufficient overexpression of the wild-type recF gene interfered with three normal cell functions: (1) UV induction of transcription from the LexA-protein-repressed sulA promoter, (2) UV resistance and (3) cell viability at 42 degrees. To show this, we altered a low-level overexpres...

Topics

Alleles
Bacterial Proteins
Base Sequence
Chromosomes, Bacterial
DNA Repair
DNA, Bacterial
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins
Gene Expression
Genes, Bacterial
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Mutation

Phenotype

Plasmids

Radiation Tolerance

Recombination, Genetic

Source

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Identifiers

PMID: 8293970
DOI: 10.1093/genetics/135.3.643
PMCID: PMC1205709

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8293970-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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