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Article

Use of high and low level overexpression plasmids to test mutant alleles of the recF gene of Escherichia coli K-12 for partial activity.

Genetics - 1 Nov 1993

Sandler S J, Clark A J

Abstract excerpt

We showed that sufficient overexpression of the wild-type recF gene interfered with three normal cell functions: (1) UV induction of transcription from the LexA-protein-repressed sulA promoter, (2) UV resistance and (3) cell viability at 42 degrees. To show this, we altered a low-level overexpressing recF+ plasmid with a set of structurally neutral mutations that increased the rate of expression of recF. The...

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Identifiers

PMID: 8293970
DOI: 10.1093/genetics/135.3.643
PMCID: PMC1205709

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