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Enhanced Measles Virus cDNA Rescue and Gene Expression after Heat Shock

1 May 1999

Abstract excerpt

Rescue of negative-stranded RNA viruses from full-length genomic cDNA clones is an essential technology for genetic analysis of this class of viruses. Using this technology in our studies of measles virus (MV), we found that the efficiency of the measles virus rescue procedure (F. Radecke et al., EMBO J. 14:5773-5784, 1995) could be improved by modifying the procedure in two ways. First, we found that coculture...

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PMID: 10196245
DOI: 10.1128/jvi.73.5.3560-3566.1999

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