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Rescue of Mumps Virus from cDNA

15 May 2000

Abstract excerpt

A complete DNA copy of the genome of a Jeryl Lynn strain of mumps virus (15,384 nucleotides) was assembled from cDNA fragments such that an exact antigenome RNA could be generated following transcription by T7 RNA polymerase and cleavage by hepatitis delta virus ribozyme. The plasmid containing the genome sequence, together with support plasmids which express mumps virus NP, P, and L proteins under control of the...

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Identifiers

PMID: 10775622
DOI: 10.1128/jvi.74.10.4831-4838.2000

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