Article
Effect of mutations of murine lens alphaB crystallin on transfected neural cell viability and cellular translocation in response to stress.
FEBS letters - 30 Oct 1998
Wiesmann K E, Coop A, Goode D, Hepburne-Scott H W, Crabbe M J
Abstract excerpt
We examined the influence of over-expressed native and mutant murine lens alphaB crystallin on the response of a murine neural cell line to heat and ionic strength shock. Native and mutant (F27R and KK174/175LL) murine alphaB crystallin amplicons were subcloned into a Lac-Switch IPTG-inducible RSV promoter eukaryotic vector, and transfected into N1E-115 cells using lipofectin. Expression was induced maximally 8 h...
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