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Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells

31 Dec 2022

Abstract excerpt

The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these...

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PMID: 36680173
DOI: 10.3390/v15010132

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