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Article

Secretory production of recombinant protein by a high cell density culture of a protease negative mutant Escherichia coli strain.

Biotechnology progress - 1 Jan 2000

Park S J, Georgiou G, Lee S Y

Abstract excerpt

Several protease negative mutant strains including HM114, HM126, and HM130 as well as their parent strain KS272 were compared for their growth and secretory production of a model fusion protein, protein A-beta-lactamase. HM114, a strain deficient in two cell envelope proteases, grew slightly faster and produced more fusion protein than the other strains deficient in more proteases. HM114 was grown to a cell dry...

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PMID: 10194390
DOI: 10.1021/bp9900108

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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