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Article

Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement.

Applied microbiology and biotechnology - 1 Mar 1995

Wittchen K D, Meinhardt F

Abstract excerpt

An efficient method for gene replacement in Bacillus megaterium was developed and used to inactivate the chromosomal neutral protease gene (nprM) from strain DSM319. A temperature-dependant suicide vector was constructed to allow replacement of the normal chromosomal copy with an altered version of the nprM gene. One mutant B. megaterium MS941 was selected for further characterization. Measurement of...

Topics

Bacillus megaterium
Bacterial Proteins
Base Sequence
Chromosome Mapping
Cloning, Molecular
DNA, Bacterial
Endopeptidases
Escherichia coli
Gene Deletion
Genes, Bacterial
Genetic Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Vectors

Molecular Sequence Data

Mutation

Plasmids

Source

Identifiers

PMID: 7766087
DOI: 10.1007/BF00191184

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7766087-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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