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Article

Q301P mutant of Vibrio PR protease affects activities under low-temperature and high-pressure conditions.

Journal of bioscience and bioengineering - 1 Oct 2020

Okai Masahiko, Onoue Chiori, Tsuda Ryo, Ishigami Chihiro, Yoshida-Mishima Chie, Urano Naoto, Kato Chiaki, Ishida Masami

Abstract excerpt

We characterized a protease of the M4 family from the cold-adapted Vibrio sp. Pr21 that was isolated from seawater at 320-m deep in Sagami Bay, Japan, and named it as PR protease based on the strain name Pr21. The PR protease had activities at 10-60 °C and 0.1-350 MPa, with the optimal temperature and pressure at 40 °C and 250 MPa. The mutant 10C9 (Q301P) obtained by error-prone PCR had higher activities than the...

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PMID: 32611521
DOI: 10.1016/j.jbiosc.2020.05.011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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