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Article

Expression of a recombinant human anti-MUC1 scFv fragment in protease-deficient Escherichia coli mutants.

International journal of molecular medicine - 1 Jun 2001

Kandilogiannaki M, Koutsoudakis G, Zafiropoulos A, Krambovitis E

Abstract excerpt

The proteolytic activity of Escherichia coli periplasmic proteases can affect the expression efficiency of many heterologous proteins such as antibody fragments that are transported to the host periplasm for folding. We investigated whether four E. coli strains that were deficient in the periplasmic proteases tsp, protease III, degP and ompT, in different combinations, affect the expression levels of an anti-MUC1...

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Source

Identifiers

PMID: 11351281
DOI: 10.3892/ijmm.7.6.659

Genes

MUC1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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