Construction and characterization of a set of E. coli strains deficient in all known loci affecting the proteolytic stability of secreted recombinant proteins.

doi:10.1038/nbt1194-1107

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Article

Construction and characterization of a set of E. coli strains deficient in all known loci affecting the proteolytic stability of secreted recombinant proteins.

Bio/technology (Nature Publishing Company) - 1 Nov 1994

Meerman H J, Georgiou G

Abstract excerpt

Even though secretion offers numerous advantages for the production of proteins in Escherichia coli, the expression of many heterologous proteins is severely limited by degradation in the periplasmic space. We found that mutations in rpoH, the RNA polymerase sigma factor responsible for heat shoc...

Topics

Chromosome Mapping
Endopeptidases
Escherichia coli
Mutation
Recombinant Proteins

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