Article
Prediction and experimental testing of Bacillus acidocaldarius thioredoxin stability.
The Biochemical journal - 15 Apr 1999
Pedone E, Cannio R, Saviano M, Rossi M, Bartolucci S
Abstract excerpt
In order to investigate further the determinants of protein stability, four mutants of thioredoxin from Bacillus acidocaldarius were designed: K18G, R82E, K18G/R82E, and D102X, in which the last four amino acids were deleted. The mutants were constructed on the basis of molecular dynamic studies and the prediction of the structure of thioredoxin from B. acidocaldarius, performed by a comparative molecular...
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