Article
The key amino acid residue of prostaglandin EP3 receptor for governing G protein association and activation steps.
Biochemical and biophysical research communications - 5 Feb 1999
Satoh S, Chang C s, Katoh H, Hasegawa H, Nakamura K, Aoki J, Fujita H, Ichikawa A, Negishi M
Abstract excerpt
To assess the role of the conserved DPWXY motif of the seventh transmembrane domain in prostanoid receptor-mediated G protein activation, we have mutated the negatively charged Asp-318 in this motif of the Gi-coupled mouse prostaglandin EP3 receptor to uncharged but polar Asn (EP3-D318N) and to the non-polar Leu (EP3-D318L). The EP3 agonist and antagonist showed similar binding affinities for the wild-type and...
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