[Development and application of the method for controlling contamination of tumor samples with normal cells to detect deletion of P16 gene in primary lung carcinoma].

Abstract excerpt

OBJECTIVE: The method is to be established to effectively promote the sensitivity of the detection of P16 gene deletion from tumor tissues mixed with normal cells and to determine the relationship between the gene alteration and the lung cancers. METHODS: The normal DNA and that of MCF-7 cells with P16 gene deletion was proportionally mixed and the mixture was limitedly diluted. The exon2 of P16 gene and internal...

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