Article
Localization and properties of a silencing element near the mat3-M mating-type cassette of Schizosaccharomyces pombe.
Genetics - 1 Mar 1999
Thon G, Bjerling K P, Nielsen I S
Abstract excerpt
Transcription is repressed in a segment of Schizosaccharomyces pombe chromosome II that encompasses the mat2-P and mat3-M mating-type cassettes. Chromosomal deletion analysis revealed the presence of a repressor element within 500 bp of mat3-M. This element acted in synergy with the trans-acting factors Swi6, Clr1, Clr2, Clr3, and Clr4 and had several properties characteristic of silencers: it did not display...
Topics
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cell Cycle Proteins
- Centromere
- Crosses, Genetic
- Escherichia coli
- Genes, Fungal
- Genes, Mating Type, Fungal
- Genotype
- Models, Biological
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Plasmids
- Schizosaccharomyces
- Transformation, Genetic