Article
Complementation of the fol2 deletion in Saccharomyces cerevisiae by human and Escherichia coli genes encoding GTP cyclohydrolase I.
Biochemical and biophysical research communications - 16 Feb 1999
Mancini R, Saracino F, Buscemi G, Fischer M, Schramek N, Bracher A, Bacher A, Gütlich M, Carbone M L
Abstract excerpt
Saccharomyces cerevisiae is so far the only organism where a knock-out mutant in the gene encoding GTP cyclohydrolase I (FOL2) has been obtained. GTP cyclohydrolase I controls the de novo biosynthetic pathway of tetrahydrobiopterin and folic acid. Since deletion of yeast FOL2 leads to a recessive auxotrophy for folinic acid, we used a yeast fol2Delta mutant for an in vivo functional assay of heterologous GTP...
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