Article
Methylene tetrahydrofolate dehydrogenase/cyclohydrolase and the synthesis of 10-CHO-THF are essential in Leishmania major.
Molecular microbiology - 1 Mar 2009
Murta Silvane M F, Vickers Tim J, Scott David A, Beverley Stephen M
Abstract excerpt
10-Formyl tetrahydrofolate (10-CHO-THF) is a key metabolite in C1 carbon metabolism, arising through the action of formate-tetrahydrofolate ligase (FTL) and/or 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase (DHCH). Leishmania major possesses single DHCH1 and FTL genes encoding exclusively cytosolic proteins, unlike other organisms where isoforms occur in the...
Topics
- Animals
- Cloning, Molecular
- Folic Acid Antagonists
- Gene Knockout Techniques
- Genes, Essential
- Genes, Protozoan
- Leishmania major
- Leucovorin
- Methenyltetrahydrofolate Cyclohydrolase
- Methylenetetrahydrofolate Dehydrogenase (NADP)
- Mutation
- Recombinant Proteins