Molecular genetic analysis of Saccharomyces cerevisiae C1-tetrahydrofolate synthase mutants reveals a noncatalytic function of the ADE3 gene product and an additional folate-dependent enzyme.

Abstract excerpt

In eucaryotes, 10-formyltetrahydrofolate (formyl-THF) synthetase, 5,10-methenyl-THF cyclohydrolase, and NADP(+)-dependent 5,10-methylene-THF dehydrogenase activities are present on a single polypeptide termed C1-THF synthase. This trifunctional enzyme, encoded by the ADE3 gene in the yeast Saccharomyces cerevisiae, is thought to be responsible for the synthesis of the one-carbon donor 10-formyl-THF for de novo...

Topics

• Aminohydrolases
• Formate-Tetrahydrofolate Ligase
• Genes, Fungal
• Immunoblotting
• Kinetics
• Methylenetetrahydrofolate Dehydrogenase (NADP)
• Molecular Weight
• Multienzyme Complexes
• Mutagenesis, Site-Directed
• Phenotype