Mutagenic alteration of the distal switch II region of RAS blocks CDC25-dependent signaling functions.

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Article

Mutagenic alteration of the distal switch II region of RAS blocks CDC25-dependent signaling functions.

The Journal of biological chemistry - 3 Jun 1994

Mirisola M G, Seidita G, Verrotti A C, Di Blasi F, Fasano O

Abstract excerpt

We have explored the role of the distal switch II region of the yeast RAS2 protein in determining the response to the nucleotide exchange factor CDC25. We first constructed yeast tester strains in which the deletion of the chromosomal CDC25, RAS1, and RAS2 genes, in combination with the chromosomal suppressor CRI4, resulted in detectable phenotypes in vivo and in vitro. Phenotypes included impaired growth at 37...

Topics

Adenylyl Cyclases
Cell Cycle Proteins
Chromosomes, Fungal
Cyclic AMP
Fungal Proteins
GTP-Binding Proteins
Genes, Fungal
Genes, Suppressor
Genotype
Glucose
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis

Plasmids

Restriction Mapping

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Species Specificity

Suppression, Genetic

Source

Open in PubMed

Identifiers

PMID: 8195227

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8195227-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Mutagenic alteration of the distal switch II region of RAS blocks CDC25-dependent signaling functions.PMID 8195227Read ↗

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