Article

Characterization of Freshly Isolated Human Peripheral Blood B Cells, Monocytes, CD4 + and CD8+ T Cells, and Skin Mast Cells by Quantitative Transcriptomics

2024-11-13

Abstract excerpt

Quantitative transcriptomics offers a new way to obtain a detailed picture of freshly isolated cells. By direct isolation, the cells are unaffected by in vitro culture and the isolation at cold temperatures maintains the cells relatively unaltered in phenotype by avoiding activation through receptor crosslinking or plastic adherence. Simultaneous analysis of several cell types provides the opportunity to obtain de...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: ff25e568-deb8-5476-8967-5fb37dd82d49
DOI: 10.20944/preprints202411.0985.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Characterization of Freshly Isolated Human Peripheral Blood B Cells, Monocytes, CD4 + and CD8+ T Cells, and Skin Mast Cells by Quantitative TranscriptomicsDOI 10.20944/preprints202411.0985.v1Read

Cultures of Human Skin Mast Cells, an Attractive In Vitro Model for Studies of Human Mast Cell BiologySemantic proximity Read

Redefinition of the human mast cell transcriptome by deep-CAGE sequencingSemantic proximity Read

Defining mast cell differentiation and heterogeneity through single-cell transcriptomics analysisSemantic proximity Read

Single-cell transcriptomics reveals the identity and regulators of human mast cell progenitorsSemantic proximity Read

Single cell transcriptome profiling reveals pathogenesis of bullous pemphigoidSemantic proximity Read

Single-cell omics-based characterization of human basophils reveals two transcriptionally distinct populationsSemantic proximity Read

The transcriptional program, functional heterogeneity, and clinical targeting of mast cellsSemantic proximity Read

Single cell transcriptome profiling reveals pathogenesis of Bullous PemphigoidSemantic proximity Read

Human Bone Marrow Plasma Cell Atlas: Maturation and Survival Pathways Unraveled by Single Cell AnalysesSemantic proximity Read

Single-cell transcriptomics reveals the identity and regulators of human mast cell progenitorsSemantic proximity Read

Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.Semantic proximity Read

Single-cell RNA sequencing identifies distinct transcriptomic signatures between PMA/ionomycin- and αCD3/αCD28-activated primary human T cellsSemantic proximity Read