Article

Understanding and evaluating ambiguity in single-cell and single-nucleus RNA-sequencing

2023-01-04

Abstract excerpt

Recently, a new modification has been proposed by Hjörleifsson and Sullivan et al . to the model used to classify the splicing status of reads (as spliced (mature), unspliced (nascent), or ambiguous) in single-cell and single-nucleus RNA-seq data. Here, we evaluate both the theoretical basis and practical implementation of the proposed method. The proposed method is highly-conservative, and therefore, unlikely to...

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Literature Corpus work: f83468ee-de3e-5f8b-8fd0-9d755b97f7c4
DOI: 10.1101/2023.01.04.522742

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