Article

Differences in molecular sampling and data processing explain variation among single-cell and single-nucleus RNA-seq experiments

2022-08-03

Abstract excerpt

A mechanistic understanding of the biological and technical factors that impact cell and nuclear transcript measurements is essential to designing, analyzing, and interpreting single-cell and single-nucleus RNA sequencing experiments. RNA sampling in nuclei and cells is fundamentally different as nuclei contain the same pre-mRNA population as cells, yet contain a small subset of the largely-cytoplasmic mRNAs. None...

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Literature Corpus work: 4bcd9587-7cdf-57ee-bc1a-8d6b1b63df9e
DOI: 10.1101/2022.08.01.502392

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