Article

Saliva direct PCR protocol for HLA-DQB1*02 genotyping

2023-10-25

Abstract excerpt

<h4>ABSTRACT</h4> Celiac Disease (CD) is an immune disorder that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor, and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also d...

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Literature Corpus work: f2300964-db92-56b7-ad78-98f1f21a9c8e
DOI: 10.1101/2023.10.25.23297535

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