Article
Saliva direct PCR protocol for HLA-DQB1*02 genotyping.
International journal of immunogenetics - 1 Apr 2024
Carrillo Angeles, Manzur María Jimena, Juri Ayub Maximiliano
Abstract excerpt
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes...
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