Article
HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Lavant Ewa H, Carlson Joyce
Abstract excerpt
The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step,...
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