Article
A population-wide applicable HLA-DQ2 and DQ8 genotyping using DNA from dried blood spots and duplex allele-specific qPCR amplification.
Scandinavian journal of clinical and laboratory investigation - 1 Nov 2016
Aguayo-Patrón Sandra, Beltrán-Sauceda Lizbeth, Calderón de la Barca Ana María
Abstract excerpt
Genotyping of HLA-DQ2 and DQ8 haplotypes is important for diagnosis or for screening of early risk detection of celiac disease or type 1 diabetes. Usually, venous blood DNA extraction and expensive and time consuming amplification are used, that hinder population-wide studies. We assayed a friendly HLA-DQ2 and DQ8 genotyping procedure using a combination of DNA from dried blood spot (DBS) and duplex...
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