Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter

Abstract excerpt

<h4>ABSTRACT</h4> Protein clustering plays numerous roles in cell physiology and disease. However, protein oligomers can be difficult to detect because they are often too small to appear as puncta in conventional fluorescence microscopy. Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS ( Clu sters M agnified by P hase S eparation). A CluMPS re...

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• Cell Death
• Corticosterone
• Fluorescence Resonance Energy Transfer
• Huntingtin Protein
• Intrinsically Disordered Proteins
• Mutation
• Nerve Tissue Proteins
• Transfection

Identifiers and source

Literature Corpus work

f0932550-d2aa-527a-9484-d043767c26f0

DOI

10.1101/2022.07.13.499962